In this study, Anacyclus pyrethrm Link (roots) is a medicinal plant from the Asteraceae family, endemic to Morocco and is widely used in traditional medicine; was investigated for their total phenolics, flavonoids contents and antioxidant activities. The quantitative estimation of total flavonoids and phenols by the colorimetric method showed that the extracts were prepared by fractionation of the crude hydromethanolic extract (ethyl acetate and n-butanol). The phenol content of various extracts, estimated by the Folin-Ciocalteu method, was between 1,44 and 12,82 mg EAG / g extract of A. pyrethrum. Moreover, it is clear that ethyl acetate was the richest fraction of total phenols in A. pyrethrum (12,82 mg EAG / g extract). The determination of flavonoids by the AlCl3 method also revealed that the fraction of ethyl acetate had the highest content (46,82 mg EQ / g of extract).
In addition, we studied the antioxidant activity of the phenolic extracts by the capacity of free radical trapping DPPH•. The results reported that the scavenger power of phenolic extracts compared to that of the standard antioxidant, ascorbic acid (IC50 = 0,051 mg / ml), allowed us to deduce that the antioxidant power of all extracts tested by A. pyrethrum studied had an important antioxidant power. The IC50 values were classified in the following order of antioxidant potency: ethyl acetate fraction (0,144 mg / ml) > hydromethanolic extract (0,152 mg / ml) > butanolic extract (0,155 mg / ml).
The results indicated that the plants tested may be potential sources for isolation of natural antioxidant compounds.
Elazzouzi, Hanane; Zekri, Nadia; Zair, Touriya; and Alaoui El Belghiti, Mohamed
"Total phenolic and flavonoid contents of Anacyclus pyrethrum Link plant extracts and their Antioxidant activity,"
Karbala International Journal of Modern Science: Vol. 5
, Article 10.
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