Entamoeba spp. in particular E.histolytica is the main reason of human amoebiasis. The molecular mechanism of its pathogenicity is poorly understood, therefore the aim this study is to investigate these mechanism on both molecular and ultrastructure levels. Tight junction (TJ) genes Claudin-1 (Cldn1) and Occludin (Ocln) were investigated by real time PCR and the pathological changes by scanning electron microscopy (SEM) and transmission electron microscope (TEM), the result showed that the gene expression levels of TJ genes were significantly high in rats infected with E. histolytica E. dispar and E. moshkovskii after 28, 14, 7 days in compare with healthy control. The expression of these genes were highest in rats infected with E. moshkovskii after 28 days, 14 and 7 day followed by E. dispar then E. histolytica .Electron microscope identified the pathogenesis of Entamoeba spp. which showed the adhesion of trophozoites and its effect on the epithelium, as well as changes in the properties of trophozoite, internal vesicles and filopodia which are very important in the adhesion and pathogenesis. Moreover, cytolysis was observed and compared among the three types.
AL-Hilfi, Athraa A. Dr; Al-Malak, Maha Khalil; Al-Ali, Shereen Jawad; and Al-Tomah, Muslim Abd-ulrahman
"Molecular and ultrastructure study of tight junction during experimental Entamoeba spp. infection,"
Karbala International Journal of Modern Science: Vol. 6
, Article 7.
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