This study investigated the toxicity of a new imidazole compound, 1-(1,4,5-triphenyl-1H-imidazol-2-yl)-naphthalen-2-ol, through the evaluation of selected oxidative stress markers and antioxidants. Both male and female D. melanogaster (3–5 days old) were fed a diet containing the imidazole derivative (20, 50, and 100 mg IMZ/kg diet) for five days. After cessation of imidazole treatment, half the population of the imidazole-exposed flies was homogenized for biochemical assays, while the other half of the flies was allowed to have a normal diet for an additional five days to see if any induced effect would be resolved. Imidazole derivatives did not significantly affect the survival rate of flies. Furthermore, there was a significant increase in reduced glutathione level (GSH) (p < 0.05) when compared to the control group. Also, the activity of catalase was significantly increased in flies fed with the 50 mg IMZ/kg diet. An increase was observed in the levels of glutathione peroxidase, glutathione transferase, total protein level, nitric oxide, and lipid peroxidation, but this elevation was not statistically significant compared to the control group. Flies fed the 100 mg IMZ/kg diet showed a significant increase in protein carbonyl level and DNA fragmentation, implying that the imidazole derivative is toxic to the flies at higher concentrations. However, this toxic effect was resolved within five days after treatment cessation. The results support the safety prospects of 1-(1,4,5-triphenyl-1H-imidazol-2-yl)-naphthalen-2-ol as an alternative anti-parasite therapy. The present findings represent a portion of the critical pre-clinical data needed to warrant further experiments and development of the imidazole-based compound as a prospective anti-parasite therapy.
OLUWAYEMI, Elizabeth Temidayo; AWAKAN, Oluwakemi Josephine; ESEOLA, Abiodun Omokehinde; PLASS, Winfried; and ADEYEMI, Oluyomi Stephen
"Imidazole derivative improves antioxidant status and causes differential alteration of redox-status in Drosophila melanogaster,"
Karbala International Journal of Modern Science: Vol. 8
, Article 4.
Available at: https://doi.org/10.33640/2405-609X.3206
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