The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e-06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (+). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.
Shehzada, Aamir; Sumartono, Christijogo; Nugraha, Jusak; Susilowatid, Helen; Wijayab, Andi Yasmin; Ahmad, Hafiz Ishfaq; Tyasningsih, Wiwiek; and Rantam, Fedik Abdul
"Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach,"
Karbala International Journal of Modern Science: Vol. 8
, Article 14.
Available at: https://doi.org/10.33640/2405-609X.3221
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.