Glioblastoma is one of the most combative astrocytoma that is resistant to chemotherapy and radiotherapy. This resistance makes it very difficult to treat. However, researches have shown that nanoparticles especially C60 hydrofullerene have antioxidant and anticancer activity. The effect of C60 hydrofullerene in cancer has been extensively studied; however, the potential regulation of autophagy and modulation of the Glial Fibrillary Acidic Protein (GFAP) gene has not been addressed in glioblastomas. Glioblastoma U-373 cell was treated with 0.5 µM of C60 hydrofullerene and/or 1 mM of hydrogen peroxide (H2O2) for 24 hours. This study demonstrated that C60 hydrofullerene and H2O2 significantly decreased cell proliferation. The gene expression and conversion of LC3-II/LC3-I were significantly induced in the separate and combined treatments. The immunofluorescent results observe that H2O2 dramatically inhibits the expression of GFAP. However, when the cells were treated with both H2O2 and C60 hydrofullerene, GFAP expression was significantly restored. The anti-proliferative effects of C60 hydrofullerene and H2O2 are shown in the results. Both treatments induced LC3-based autophagy and H2O2 was down regulated GFAP.
Faraj, Aryan M.; Agca, Can A.; Nedzvetsky, Victor S.; and Tykhomyrov, Artem A.
"C60 Hydrofullerene Induced Autophagy and Ameliorated GFAP In H2O2 Treated Human Malignant Glioblastoma U-373 Cell Line,"
Karbala International Journal of Modern Science: Vol. 8
, Article 19.
Available at: https://doi.org/10.33640/2405-609X.3242
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.