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Abstract

Cervical cancer has a high incidence rate in women, making the development of cancer drugs increasingly urgent. Asian swamp eel protein hydrolysate shows great promise as an anticancer candidate. This study aimed to reveal the potential of Asian swamp eel protein hydrolysate as an anticancer candidate in the cervix. Asian swamp eel protein hydrolysate was produced by hydrolyzing flesh with the enzyme alcalase. Compounds and peptides were identified using LC-HRMS. These compounds and peptides were analyzed using network pharmacology, gene ontology, and molecular docking. The results identified 153 endogenous compounds, including fatty acids and peptides. The key proteins targeted by the hydrolysate included HDAC1, STAT3, AKT1, EGFR, and MMP9. The signaling pathways involved included the renin-angiotensin system, the VEGF signaling pathway, and the apoptosis pathway. Biological processes showed potential to regulate blood vessels and disassemble the extracellular matrix that lies between cell membranes and the extracellular region through various peptide receptor activities. The highest activity was observed with the cyclo(Phenylalanine-Glutamine) compound, which inhibited MMP9 and EGFR with a binding affinity of -7.3kcal/mol. This research contributes to the discovery of new anticancer compounds. It highlights the importance of computational methods in accelerating the development of therapeutic agents, but further testing is needed to rein-force these findings with in vitro and in vivo approaches.

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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