Immuno-Bioinformatics Study of Secondary Metabolites from Watercress Nasturtium officinale as Immunostimulant in Whiteleg Shrimp Litopenaeus vannamei by Targeting Peroxinectin and Scavenger Receptor Class B

Qurrota A’yunin, Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, IndonesiaFollow
Muhaimin Rifa’i, Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia
Maftuch Maftuch, Aquaculture Department, Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia
Yoga Dwi Jatmiko, Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia
Muhammad Hermawan Widyananda, Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia
Triyanto Triyanto, Fisheries Department, Faculty of Agriculture, Universitas Gajah Mada, Yogyakarta, Indonesia.
Muhammad Tegar Handrianto, Aquaculture Department, Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Abstract

The viral disease affects the whiteleg shrimp (Litopenaeus vannamei) and causes losses. Immunostimulants are recognized as a more environmentally friendly approach to disease management and antimicrobial properties of aquatic organisms. Watercress (Nasturtium officinale) is an aquatic plant species that contains antioxidant properties that can protect the organism from disease and boost the immune system. Shrimp rely heavily on innate immunity to combat infectious agents due to their absence of adaptive immunity. Peroxinectin and scavenger receptor class B (SRB) play essential roles in the shrimp defense system. This research employs a bioinformatic to assess the immunostimulatory potential of watercress by targeting peroxinectin and SRB in L. vannamei. Based on LC-HRMS analysis, the best value >90% M/Z of watercress was identified in 7 compounds. Protein homology modeling showed a large number of amino acid residues in the favored region, peroxinectin at 95.12% and SRB at 99.40%. Rutin, one of the 7 identified compounds, has the lowest value of binding affinity, peroxinectin at -9.8 (kcal/mol) and SRB at -7.1 (kcal/mol). Rutin interaction with receptor proteins forms the most hydrogen bonds among other compounds. The RMSF value of both receptor proteins remained below 2.5Å, implying the sustained stability and cohesion of the ligand targets throughout the simulation. The bioactive compound of watercress N. officinale, especially rutin, has shown stable binding to peroxinectin and SRB. This indicates that the compound is potentially an immunostimulant to activate receptor protein, peroxinectin, and scavenger receptor class B and combat viral infection

Recommended Citation

A’yunin, Qurrota; Rifa’i, Muhaimin; Maftuch, Maftuch; Jatmiko, Yoga Dwi; Widyananda, Muhammad Hermawan; Triyanto, Triyanto; and Handrianto, Muhammad Tegar (2025) "Immuno-Bioinformatics Study of Secondary Metabolites from Watercress Nasturtium officinale as Immunostimulant in Whiteleg Shrimp Litopenaeus vannamei by Targeting Peroxinectin and Scavenger Receptor Class B," Karbala International Journal of Modern Science: Vol. 11 : Iss. 1 , Article 13.
Available at: https://doi.org/10.33640/2405-609X.3395