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Abstract

Silver nanoparticles (AgNPs) were synthesized via an innovative green synthesis method using amygdalin (Am) as a reducing agent and the Nd: YAG laser as a catalyst. We studied the properties of the nanoparticles using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX), atomic force microscopy (AFM), ultraviolet-visible spectroscopy (UV), and Fourier transform infrared spectroscopy (FTIR) techniques. All the results of the examination demonstrate excellent structural and optical properties. In addition, the molecular docking of the complex composed of amygdalin and AgNPs was tested on three proteins concerned with the virulence of Pseudomonas aeruginosa and their resistance to antibiotics. These proteins, 4QU3, 7MQK, and 2UWJ had -6.82, -6.64, and -6.41 binding affinity (kcal/mol), respectively. The antimicrobial activity was tested on multi-drug-resistant (MDR) P. aeruginosa isolates from wounds and burns, confirming their potential as effective antibacterial agents, with the inhibition rate reaching (20.73 mm) for wound isolations at a concentration of (5 mM) for Am-AgNPs. A cytotoxicity test (MTT) was tested for (24 h) on human dermal fibroblasts (HDFn) and human melanoma cells (A375). Compared to the normal cell line, the outcomes against the cancer line were exceptional. The objective of this research is to rapidly produce AgNPs in an environmentally favorable manner. The therapeutic efficacy of Am-AgNPs against MDR P. aeruginosa isolated from wounds and burns, as well as the synthetic nanomaterial's efficacy against the skin cancer line, creates new opportunities for the treatment of intractable diseases caused by bacteria that are resistant to antibiotics, in addition to cancer.

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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