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Abstract

The food sector must contend with issues such as pathogen resistance to some of the available chemical agents, environmental pollution, and climate change to provide healthy food for livestock and people. The application of atmospheric pressure plasma jets (APPJs) is one potential solution for such problems. Plasma is appropriate for effective surface decontamination regarding food products and seeds, surface decontamination, and achieving improved agricultural production yields. The impact of plasma-activated water (PAW) produced by plasma jet discharge (PJD) system on in vitro-cultivated wheat seeds is examined in this work. For this aim, a plasma jet system was constructed with a total power of 60W, a frequency of 20kHz, and an AC voltage sine wave ranging between 0 and 12 kVA from peak to peak. Through directly activating 60 cc of distilled water (DW) with 20 cc of plasma containing varying quantities of reactive oxygen as well as nitrogen species (RONS) and pH, such method uses 2.5 liters of argon gas every minute. To produce PAW, DW has been exposed to PJD for 20 minutes. Three hundred wheat seeds have been tagged and split equally into five groups for this study. Active water was applied to three groups of seeds. The last group received simply DW treatment, the fourth group's seeds were placed in DW before being exposed directly to plasma. In PAW, the seeds were soaked for a whole day. Following a 24-hour period, fifteen germination plates—three for each group—were used to plant the seeds. Following soaking, the seeds were observed for three days, and the dishes were placed in a dry room at room temperature and administered DW as directed. Monitoring was done using germination indicators and parameters, such as the germination rate (GR) and the percentage of germination (Gp). Following its plasma treatment, DW resulted in notable modifications to its chemical parameters and physical characteristics. In the case when seeds were soaked in plasma-treated water, the rate of germination (metrics of growth, like shoot/seedling length as well as seeds’ imbibition rate) rose. The seeds that were solely soaked in DW produced a higher germination yield. Since RONS in PAW interacted directly with the surface of the seed, increasing the germination rate and water and nutrient uptake, group S4 produced the greatest results when the seeds have been directly exposed to plasma..

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Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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