Antifungal Peptides from Casein Milk of Etawa Crossbreed (Capra hircus) as Biopreservation Agent for Bread and Molecular Docking Studies

Authors

Dian Riana Ningsih, Department of Chemistry, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Winarto Haryadi, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
Rachma Wikandari, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Tri Joko Raharjo, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, IndonesiaFollow

Abstract

Bread without peptide fraction treatment started to grow mold on the fourth day with a total of 24x10⁷ fungi colonies/ml. Meanwhile, the addition of peptide fractions GMC5, GMC6, GMC7, and GMC8 effectively preserved bread for up to 4 days, indicated by no mold growth. The best treatment was the GMC6 peptide fraction on day 8 which grew the least amount of fungus at 7x10⁷ spores/ml. Peptide interaction with the CaATPase receptor Aspergillus sp. was carried out using HADDOCK 2.4. The 3D CaATPase structure prediction resulted in a model with good structural quality. This was supported by residues in favored regions at 99.53%, a 3D-1D average score ≥ 0.2 with a percentage of 81.05%, and a Molprobity score of 1.10. The four peptides exhibited great potential in inhibiting CaATPase through molecular binding with consecutive affinity energies of -6.7, -8.2, -7.7, and -5.6 kcal/mol for YNVPQLEIVPK, KENINELSK, GLSPEVPNENLLR, and YLGYLEQLLK, respectively. These peptides have the potential as bread preservatives

Recommended Citation

Ningsih, Dian Riana; Haryadi, Winarto; Wikandari, Rachma; and Raharjo, Tri Joko (2025) "Antifungal Peptides from Casein Milk of Etawa Crossbreed (Capra hircus) as Biopreservation Agent for Bread and Molecular Docking Studies," Karbala International Journal of Modern Science: Vol. 11 : Iss. 3 , Article 18.
Available at: https://doi.org/10.33640/2405-609X.3425

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