Evaluation of MCF-7 Breast Cancer Cell Cytotoxic and Antioxidant Activities of Peptide Fractions from Symbiotic Bacteria of Jellyfish Catostylus sp.

Eka Sry Wahyuni, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, IndonesiaFollow
Ahyar Ahmad, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, IndonesiaFollow
Muhammad Nasrum Massi, Department of Clinical Microbiology, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, Indonesia.Follow
Sofa Fajriah, Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), South Tangerang, Banten, IndonesiaFollow
Randi Rimpung, Doctoral Program, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, IndonesiaFollow
Muh. Akbar Ardiputra, Master’s Program in Biomedical Sciences, Postgraduate School, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, IndonesiaFollow
Harningsih Karim, Department of Pharmacy, School of Pharmacy, Ma’bullo Sibatang Educational Foundation, Makassar 90222, IndonesiaFollow
Irda Handayani, Department of Clinical Pathology, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, IndonesiaFollow

Abstract

Marine-derived symbiotic microorganisms are recognized as a promising source of bioactive compounds with potential therapeutic applications, yet research on jellyfish-associated bacteria remains limited. This study examines the bioactivity of peptide fractions derived from symbiotic bacteria isolated from the jellyfish Catostylus sp., collected from the coastal waters of South Sulawesi, Indonesia, with a focus on their anticancer and antioxidant properties. Following sample collection, the symbiont bacteria were isolated, enzymatically hydrolyzed, and purified before their biological activity was evaluated. Preliminary cytotoxicity screening using the brine shrimp lethality assay revealed that the extracellular peptide fraction (5–10 kDa) and intracellular peptide fraction (3–5 kDa) exhibited the highest cytotoxic effects. Subsequent testing against MCF-7 breast cancer cells demonstrated potent anticancer activity, with IC₅₀ values of 19.83 µg/mL (5–10 kDa) and 6.43 µg/mL (3–5 kDa). Antioxidant assays using the DPPH and ABTS methods showed that the 5–10 kDa fraction achieved IC₅₀ values of 254.25 µg/mL (DPPH) and 41.30 µg/mL (ABTS), whereas the 3–5 kDa fraction recorded IC₅₀ values of 218.02 µg/mL (DPPH) and 37.89 µg/mL (ABTS). These findings suggest that peptide fractions from jellyfish-associated symbiotic bacteria exhibit significant in vitro anticancer and antioxidant properties. Although the present study is limited to in vitro analysis, it provides novel evidence supporting the potential of Catostylus sp.-derived bacterial peptides as candidates for the development of antioxidant-based therapies for cancer prevention and treatment, particularly breast cancer

Recommended Citation

Wahyuni, Eka Sry; Ahmad, Ahyar; Massi, Muhammad Nasrum; Fajriah, Sofa; Rimpung, Randi; Ardiputra, Muh. Akbar; Karim, Harningsih; and Handayani, Irda (2026) "Evaluation of MCF-7 Breast Cancer Cell Cytotoxic and Antioxidant Activities of Peptide Fractions from Symbiotic Bacteria of Jellyfish Catostylus sp.," Karbala International Journal of Modern Science: Vol. 12 : Iss. 1 , Article 10.
Available at: https://doi.org/10.33640/2405-609X.3442