In Vivo Expression Profiling of Listeria monocytogenes Internalins (inlF, inlP, inlJ) and Their Corresponding Host Receptors (VIM, AFDN, MUC2) Using a Rabbit Ligated Intestinal Loop Model

Nawar Al-Janabi, Department of Medical Biotechnology, College of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq.Follow
Nadhim Mushtaq Hashim Al-bderee, Department of Medical Biotechnology, College of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq
Zahraa M. Madania, Department of Medical Biotechnology, College of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq.
Mohanad Jawad Kadhim, Department of Applied Biotechnology, College of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq.

Abstract

Listeria monocytogenes is a Gram-positive pathogen that causes severe infections, particularly in immunocompromised individuals. Internalin genes are important virulence factors that facilitate the bacterium's invasion of host cells. This research aimed to quantify in vivo expression of selected internalin genes (inlF, inlP, and inlJ) and their corresponding host receptors (VIM, AFDN, and MUC2) during L. monocytogenes infection. To achieve this aim, a rabbit ligated intestinal loop model was constructed comprising two intestinal loops. The first loop was injected with 1 mL of L. monocytogenes (1 × 10⁷ CFU/mL), while the second loop was injected with 1 mL of PBS as a control. The relative gene expression was determined using RT-qPCR. Results showed that, in contrast to L. monocytogenes grown on BHI agar (in vitro control), the expression of inlF, inlP, and inlJ was upregulated significantly in L. monocytogenes injected into intestinal loops with fold changes of 5.82 (p

Recommended Citation

Al-Janabi, Nawar; Al-bderee, Nadhim Mushtaq Hashim; Madania, Zahraa M.; and Kadhim, Mohanad Jawad (2026) "In Vivo Expression Profiling of Listeria monocytogenes Internalins (inlF, inlP, inlJ) and Their Corresponding Host Receptors (VIM, AFDN, MUC2) Using a Rabbit Ligated Intestinal Loop Model," Karbala International Journal of Modern Science: Vol. 12 : Iss. 3 , Article 8.
Available at: https://doi.org/10.33640/2405-609X.3472