Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

Authors

Aamir Shehzada, Virology and Immunology, Division of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Christijogo Sumartono, Anaesthesiology and Reanimation Department, Dr. Soetomo General Hospital and Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
Jusak Nugraha, Clinical Pathology Department, Dr. Soetomo General Hospital and Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Helen Susilowatid, Research Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya
Andi Yasmin Wijayab, Research Centre for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
Hafiz Ishfaq Ahmad, Department of Animal Breeding and Genetics, the University of Veterinary and Animal Sciences, Ravi Campus, Pattoki, Punjab, Pakistan
Wiwiek Tyasningsih, Division of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Fedik Abdul Rantam, Virology and Immunology, Division of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Follow

Abstract

The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e-06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (+). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.

Recommended Citation

Shehzada, Aamir; Sumartono, Christijogo; Nugraha, Jusak; Susilowatid, Helen; Wijayab, Andi Yasmin; Ahmad, Hafiz Ishfaq; Tyasningsih, Wiwiek; and Rantam, Fedik Abdul (2022) "Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach," Karbala International Journal of Modern Science: Vol. 8 : Iss. 2 , Article 14.
Available at: https://doi.org/10.33640/2405-609X.3221

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