In vitro antioxidant, anti-inflammatory, and photoprotective activities of aqueous extract of the endemic plant Hammada scoparia L. from Algeria

Benine Chaima, Department of Cellular and Molecular Biology, Faculty of Life and Natural Science, University of El Oued39000, AlgeriaFollow
Djahra Ali Boutlelis, Department of Cellular and Molecular Biology, Faculty of Life and Natural Science, University of El Oued39000, Algeria
Laiche Ammar Touhami, Department of Biology, Faculty of Life and Natural Science, University of El Oued39000, Algeria
Tlili Hajer, Laboratory of Pastoral Ecosystems and Valorization of Spontaneous Plants and Microorganisms, Institute of Arid Regions (IRA), Medenine 4119, Tunisia
Tliba Ali, Laboratory of Valorisation and Technology of Sahara Resources (VTRS), University of El Oued, El-Oued 39000, Algeria
Ben Arfa Abdelkarim, Laboratory of Pastoral Ecosystems and Valorization of Spontaneous Plants and Microorganisms, Institute of Arid Regions (IRA), Medenine 4119, Tunisia
Najjaa Hanen, Laboratory of Pastoral Ecosystems and Valorization of Spontaneous Plants and Microorganisms, Institute of Arid Regions (IRA), Medenine 4119, Tunisia

Abstract

This study aimed to analyze the chemical profile and evaluate the biological activities of the aqueous extract of H. Scoparia, an endemic plant found in Southeastern Algeria. The aqueous extract was subjected to phytochemical screening, RP-HPLC, and FT-IR analysis. The anti-inflammatory activity was examined using the protein denaturation method, and the photoprotective activity was evaluated using UV-Visible spectrophotometry. The antioxidant activity was assessed using ABTS, DPPH, and FRAP assays. The study found the presence of various secondary metabolites such as polyphenols, tannins, and saponins. This study suggests that H. Scoparia extract has the potential as a natural source of bio-actives for developing drugs. The extract exhibited significant antioxidant activity. The study revealed that H. Scoparia extract demonstrated excellent photoprotective activity against UV, and significant biological activities in vitro.

Recommended Citation

Chaima, Benine; Boutlelis, Djahra Ali; Touhami, Laiche Ammar; Hajer, Tlili; Ali, Tliba; Abdelkarim, Ben Arfa; and Hanen, Najjaa (2023) "In vitro antioxidant, anti-inflammatory, and photoprotective activities of aqueous extract of the endemic plant Hammada scoparia L. from Algeria," Karbala International Journal of Modern Science: Vol. 9 : Iss. 3 , Article 17.
Available at: https://doi.org/10.33640/2405-609X.3310